IBS ANIMAL MODELS
In the same study, we observed in a model of rats having a visceral hypersensitivity induced by intrarectal instillation of high concentration of butyrate (mimicking the IBS visceral hypersensitivity without any sign of inflammation).
We also noted that the same probiotic reduces significantly the pain perception and is as efficient as the injection of morphine at the dosage of 0.1mg/kg. We demonstrated that the mechanism of action of this probiotic had impacted the expression of cannabinoid and opioid receptors.
Validated in vivo model
We commonly use a validated in vivo model to evaluate the effects of prebiotics, probiotics or other compounds in the intestinal well-being or in the visceral pain (induced by butyrate) mimicking the features of IBS that is the colorectal distension and not an invasive method. The colonic pain perception in rats can be assessed using a balloon inserted intra-rectally and inflated by a barostat system.
The tools associated to evaluate the properties of tested substances are:
- modification of the fecal flora by conventional bacteriologic methods.
- expression of the therapeutic target at the mRNA level (Real-Time PCR) and at the protein level (IHC, Western Blot).